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KMID : 0861020130280060155
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Korea Journal of Herbology
2013 Volume.28 No. 6 p.155 ~ p.160
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Houttuynia cordata Thunberg exhibits anti-tumorigenic activity in human gastric cancer cells
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Hong Se-Chul
Eo Hyun-Ji
Song Hoon-Min
Woo So-Hee
Kim Mi-Kyeong
Lee Jin-Wook
Seo Jeong-Min
Park Su-Bin
Eom Jung-Hye
Jeong Jin-Boo
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Abstract
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Objectives: Gastric cancer is a leading cause of cancer-related deaths, worldwide. Houttuynia cordata Thunberg (H. cordata) has been used as a medicinal plants and it has an anti-cancer activity in human colorectal cancer and leukemic cancer. However, the potential anti-cancer activity and mechanisms of H. cordata for human gastric cancer cells have not been tested so far. Thus, this study examined the biological effects of H. cordata on the human gastric cancer cell line SNU-1 and AGS.
Methods: Inhibition of cell proliferation and cell cycle by H. cordata was carried out by MTT assay and Muse cell cycle analysis and the expressions of protein associated with apoptosis and cell cycle regulation were investigated with Western blot analysis.
Results: In MTT assay, the proliferation of SNU-1 and AGS cells was significantly inhibited by H. cordata in a time and dose dependent manner, Inhibition of cell proliferation by H. cordata was in part associated with apoptotic cell death, as shown by changes in the expression ratio of Bax to Bcl-2 by H. cordata. Also, H. cordata regulated the expression of cell cycle regulatory proteins such as pRb, cyclin D1, cyclin E, CDK4, CDK2, p21 and p15.
Conclusion: The antiproliferative effect of H. cordata on SNU-1 and AGS gastric cancer cells revealed in this study suggests that H. cordata has intriguing potential as a chemopreventive or chemotherapeutic agent.
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KEYWORD
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Anti-cancer activity, Apoptosis, Human gastric cancer, Houttuynia cordata Thunberg
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